Additional information
Specifity
Using DL-Homocysteine-BSA conjugate (Hcys-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
| DL-Homocysteine-BSA | Kd around 10-8 M | very high affinity and specificity |
Low affinity and no crossreactivity with close competitors:
| Competitors | Affinity compared to Hcys-BSA | |
|---|---|---|
| Cysteine-BSA | 200 times lower | low affinity |
| DL-Homocysteic acid-BSA | 50 000 times lower | no crossreactivity |
The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).
Additional Information
- Host species
Rabbit
- Clonality
Polyclonal
- Sub-categories
Amino acids & related compounds
- Isotype
IgG
- Target
Conjugated DL-Homocystein
- Immunogen
Synthetic DL-Homocysteine conjugated to bovine serum albumin (BSA)
- Species reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule
- Purity
Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation
- Form
Lyophilized
- Stabilizers / preservatives
None
- Storage instructions and buffer
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles
- Research areas
Biochemistry, Neurobiology, Neurodegenerative diseases, Radicalar processes
- Applications
ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC
- Corresponding antigen/related product
To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: DL-Homocysteine conjugate (Product number: AG035)
- Related antibodies
Polyneuros also sells the polyclonal antibodies against another molecule involved in stroke: anti-Folic acid antibody (AP099)