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Phenylalanine antibody – AP039

Polyclonal anti-conjugated Phenylalanine antibody (rabbit)

SKU: AP039 Category:

From 180

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Additional information


Using Phenylalanine-BSA conjugate (Phe-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Phenylalanine-BSA Kd around 10-8 M very high affinity and specificity

No crossreactivity with close competitors:

Competitors Affinity compared to Phe-BSA
L-Tyrosine-BSA 50 000 times lower no crossreactivity
L-DOPA-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species





Amino acids & related compounds




Conjugated Phenylalanine


Synthetic Phenylalanine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule


Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation



Stabilizers / preservatives


Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Pharmacology


ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Phenylalanine conjugate (Product number: AG039)

Related antibodies

Polyneuros also sells antibodies against small molecules of the Phenylalanine pathway: anti-Dopamine (AP001 or AM001), anti-L-DOPA (AP004), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Octopamine (AP007), anti-Tyramine (AP008), anti-L-Tyrosine (AP010). Polyneuros sells also antibodies against other aminoacids: anti-L-Tyrosine (AP010), anti-L-Tryptophan (AP021), anti-Glycine (AP028), anti-Glutamate (AP029 or AM003), anti-D-Serine (AP041 or AM010)