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L-Glutamate antibody – AM003

Monoclonal anti-conjugated L-Glutamate (Glutamic acid) antibody (mouse)

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Additional information


Using L-Glutamate-BSA conjugate (Glu-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

L-Glutamate-BSA Kd around 10-8M very high affinity and specificity

Low affinity or no crossreactivity with close competitors:

Competitors Affinity compared to Glu-BSA
D-Glutamate-BSA 100 times lower low affinity
L-Aspartate-BSA 50 000 times lower no crossreactivity
GABA-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information


Monoclonal antibody was obtained after BALB/c mouse immunisation with the conjugate: L-Glutamate-bovine serum albumin (BSA). Hybridoma clone was obtained by hybridization of spleen cells with the myeloma cell line SP2/O/Ag14

Host species




Clone number



Amino acids & related compounds


IgM, Kappa


Conjugated L-Glutamate


Synthetic L-Glutamate conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule


Monoclonal antibody was preadsorbed on protein carriers and purified by ammonium sulfate precipitation



Stabilizers / preservatives


Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Neurodegenerative diseases, Pharmacology


ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: L-Glutamate conjugate (Product number: AG029)

Related antibodies

Polyneuros also sells the Polyclonal anti-L-Glutamate antibodies (AP029). Polyneuros sells as well polyclonal or monoclonal antibodies against other neurotransmitters: anti-Dopamine (AP001 or AM001), anti-Serotonin (AP014), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-GABA (AP027), anti-Glycine (AP028), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)