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Dopamine antibody – AP001

Polyclonal anti-conjugated Dopamine antibody (rabbit)

From 180

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Additional information


Using Dopamine-BSA conjugate (DA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Dopamine-BSA Kd around 10-8M very high affinity and specificity

Very low affinity with close competitors:

Competitors Affinity compared to DA-BSA
Noradrenaline-BSA 200 times lower low affinity
L-DOPA-BSA 400 times lower low affinity
Octopamine-BSA 400 times lower low affinity
Tyramine-BSA 5000 times lower very low affinity
Adrenaline-BSA 5000 times lower very low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation)

Additional Information

Host species









Conjugated Dopamine


Synthetic Dopamine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule


Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation



Stabilizers / preservatives


Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Pharmacology


ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Dopamine conjugate (Product number: AG001)

Related antibodies

Polyneuros also sells the monoclonal anti-Dopamine antibodies (AM001). Polyneuros sells as well antibodies against small molecules of the Phenylalanine pathway: anti-L-DOPA (AP004), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Octopamine (AP007), anti-Tyramine (AP008), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Polyneuros sells as well antibodies against other neurotransmitters: anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)

Clone number