Additional information
Specifity
Using DL-Octopamine-BSA conjugate (Octo-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
| DL-Octopamine-BSA | Kd around 10-8 M | Very high affinity and specificity |
Very low affinity or no crossreactivity with close competitors:
| Competitors | Affinity compared to Octo-BSA | |
|---|---|---|
| Tyramine-BSA | 400 times lower | low affinity |
| Noradrenaline-BSA | 10 000 times lower | no crossreactivity |
| Tyrosine-BSA | 50 000 times lower | no crossreactivity |
| Dopamine-BSA | 50 000 times lower | no crossreactivity |
The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).
Additional Information
- Host species
Rabbit
- Clonality
Polyclonal
- Sub-categories
Catecholamines
- Isotype
IgG
- Target
Conjugated DL-Octopamine
- Immunogen
Synthetic DL-Octopamine conjugated to bovine serum albumin (BSA)
- Species reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule
- Purity
Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation
- Form
Lyophilized
- Stabilizers / preservatives
None
- Storage instructions and buffer
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles
- Research areas
Biochemistry, Neurobiology, Pharmacology
- Applications
ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC
- Corresponding antigen/related product
To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Octopamine conjugate (Product number: AG007)
- Related antibodies
Polyneuros also sells the polyclonal and monoclonal antibodies against other molecules of Phenylalanine pathway: anti-Dopamine (AP001 or AM001), anti-L-DOPA (AP004), anti-Noradrenaline (Norepinephrine) (AP006), anti-Tyramine (AP008), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Polyneuros sells as well antibodies against other neurotransmitters: anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)