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Tyramine antibody – AP008

Polyclonal anti-conjugated Tyramine antibody (rabbit)

From 180

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Additional information


Using Tyramine-BSA conjugate (TA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Tyramine-BSA Kd around 10-8 M very high affinity and specificity

Low affinity and no crossreactivity with close competitors:

Competitors Affinity compared to TA-BSA
Octopamine-BSA 800 times lower low affinity
Dopamine-BSA 40 000 times lower no crossreactivity
L-Tyrosine-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species









Conjugated Tyramine


Synthetic Tyramine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule


Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation



Stabilizers / preservatives


Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Pharmacology


ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Tyramine conjugate (Product number: AG008)

Related antibodies

Polyneuros also sells the polyclonal and monoclonal antibodies against other molecules of Phenylalanine pathway: anti-Dopamine (AP001 or AM001), anti-L-DOPA (AP004), anti-Noradrenaline (Norepinephrine) (AP006), anti-Octopamine (AP007), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Polyneuros sells as well antibodies against other neurotransmitters: anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)