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Serotonin (5-Hydroxytryptamine) antibody – AP014

Polyclonal anti-conjugated 5-Hydroxytryptamine antibody (rabbit)

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Additional information

Specifity

Using 5-hydroxytryptamine-BSA conjugate (5-HT-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

5-Hydroxytryptamine-BSA Kd around 10-8M very high affinity and specificity

Low affinity with close competitors:

Competitors Affinity compared to 5-HT-BSA
5-Methoxytryptamine-BSA 25 times lower low affinity
Tryptamine-BSA 50 times lower low affinity
5-Hydroxytryptophan-BSA 175 times lower low affinity
5-Hydroxyptryptamine free 1150 times lower low affinity
L-Tryptophan-BSA 3500 times lower low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Indolamines

Isotype

IgG

Target

Conjugated 5-Hydroxytryptamine

Immunogen

Synthetic 5-Hydroxytryptamine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Neurodegenerative diseases, Pharmacology

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: 5-Hydroxytryptamine conjugate (Product number: AG014)

Related antibodies

Polyneuros also sells antibodies against small molecules linked to the Tryptophan pathway: anti-L-tryptophane (AP021), anti-Melatonin like (AP123). Polyneuros sells as well antibodies against other neurotransmitters: anti-Dopamine (AP001 or AM001), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029 or AM003), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)