Additional information
Specifity
Using Acetylcholine-like-BSA conjugate (ACh-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
| Acetylcholine-like-BSA | Kd around 10-8 M | very high affinity and specificity |
No crossreactivity with close competitors:
| Competitors | Affinity compared to ACh-BSA | |
|---|---|---|
| Phosphatidylcholine free | 10 000 times lower | no crossreactivity |
| Choline free | 10 000 times lower | no crossreactivity |
| Acetylcholine free | 10 000 times lower | no crossreactivity |
The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).
Additional Information
- Host species
Rabbit
- Clonality
Polyclonal
- Sub-categories
Other Neurotransmitters
- Isotype
IgG
- Target
Conjugated Choline (Acetylcholine-like)
- Immunogen
Synthetic Choline conjugated (Acetylcholine-like) to bovine serum albumin (BSA)
- Species reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule
- Purity
Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation
- Form
Lyophilized
- Stabilizers / preservatives
None
- Storage instructions and buffer
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles
- Research areas
Biochemistry, Neurobiology, Neurodegenerative diseases, Pharmacology
- Applications
ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC
- Corresponding antigen/related product
To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Choline (Acetylcholine-like) conjugate (Product number: AG049)
- Related antibodies
Polyneuros also sells the monoclonal anti-Acetylcholine-like antibodies (AM002). Polyneuros sells as well antibodies against other neurotransmitters: anti-Dopamine (AP001 or AM001), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029 or AM003), anti-Histamine (AP032 or AM005)