You cannot find what you are looking for in our existing antibodies catalogue, please check the availability of our out-of-catalog antibodies

Indole-3-Acetic acid (Auxine) antibody – AP118

Polyclonal anti-conjugated Indole-3-Acetic acid (Auxin) antibody (rabbit)

SKU: AP118 Category:

From 180

Clear

If you want, add this corresponding antigen to your cart:

For another size, request a quote

Additional information

Specifity

Using Indole-3-acetic acid-BSA conjugate (IAA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Indole-3-Acetic acid-BSA Kd around 10-8 M very high affinity and specificity

No crossreactivity with close competitors:

Competitors Affinity compared to IAA-BSA
Indole-3-Pyruvic acid-BSA 600 times lower low affinity
5-Hydroxyindole-BSA 50 000 times lower no crossreactivity
Indole-BSA 50 000 times lower no crossreactivity
Oxindole-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Target

Conjugated Indole-3-acetic acid

Immunogen

Synthetic Indole-3-acetic acid conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Plant physiology

Applications

ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Indole-3-acetic acid conjugate (Product number: AG118)

Related antibodies

Polyneuros sells also antibodies against another plant compnent: anti-Salicylate antibody (AP168)