Additional information
Specifity
Using L-Kynurenine -BSA conjugate (KYNU-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
| L-Kynurenine-BSA | Kd around 10-8 M | very high affinity and specificity |
Low affinity or no crossreactivity with close competitors:
| Competitors | Affinity compared to KYNU-BSA | |
|---|---|---|
| D-Kynurenine-BSA | 7 times lower | high affinity |
| Picolinic Acid-PC | 3 000 times lower | low affinity |
| Kynurenic acid-BSA | 50 000 times lower | no crossreactivity |
| 3-Hydroxy-Kynurenine-BSA | 50 000 times lower | no crossreactivity |
| Xanthurenic acid-BSA | 50 000 times lower | no crossreactivity |
| Quinaldic acid-BSA | 50 000 times lower | no crossreactivity |
| L-Tryptophan-BSA | 50 000 times lower | no crossreactivity |
| Quinolinic acid-BSA | 50 000 times lower | no crossreactivity |
| Anthranilic acid-BSA | 50 000 times lower | no crossreactivity |
| L-kynurenine free | 50 000 times lower | no crossreactivity |
The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).
Additional Information
- Description
Monoclonal antibody was obtained after BALB/c mouse immunisation with the conjugate: L-Kynurenine-bovine serum albumin (BSA). Hybridoma clone was obtained by hybridization of spleen cells with the myeloma cell line SP2/O/Ag14
- Host species
Mouse
- Clonality
Monoclonal
- Clone number
KYNU4A6
- Sub-categories
IDO compounds
- Isotype
IgG1, Kappa
- Target
Conjugated L-Kynurenine
- Immunogen
Synthetic L-Kynurenine conjugated to bovine serum albumin (BSA)
- Species reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule
- Purity
Monoclonal antibody was preadsorbed on protein carriers and purified by ammonium sulfate precipitation
- Form
Lyophilized
- Stabilizers / preservatives
None
- Storage instructions and buffer
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles
- Research areas
Neurobiology, Neurodegenerative diseases
- Applications
ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC
- Corresponding antigen/related product
To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: L-Kynurenine conjugate (Product number: AG147)
- Related antibodies
Polyneuros also sells the polyclonal anti-L-Kynurenine antibody (AP147). Polyneuros sells as well polyclonal antibodies against other small molecules of the IDO pathway: anti-Kynurenic acid (AP062), anti-Quinolinic acid (AP063)