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DL-Homocysteic acid antibody – AP036

Polyclonal anti-conjugated DL-Homocysteic acid antibody (rabbit)

SKU: AP036 Category:

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Additional information

Specifity

Using DL-Homocysteic acid-BSA conjugate (HcysA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

DL-Homocysteic acid-BSA Kd around 10-8 M very high affinity and specificity

Low affinity with close competitors:

Competitors Affinity compared to HcysA-BSA
L-Glutamate-BSA 1 000 times lower low affinity
L-Aspartate-BSA 1 000 times lower low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Oxidative & Lipoperoxydative metabolites

Isotype

IgG

Target

Conjugated DL-homocysteic acid

Immunogen

Synthetic DL-Homocysteic acid conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Neurodegenerative diseases, Radicalar processes

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: DL-Homocysteic acid conjugate (Product number: AG036)

Related antibodies

Polyneuros also sells another Oxidative & Lipoperoxydative metabolite: anti-Malondialdehyde (AP050)