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L-Glutathione antibody – AP047

Polyclonal anti-conjugated L-Glutathione antibody (rabbit)

SKU: AP047 Category:

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Additional information

Specifity

Using L-Glutathione-BSA conjugate (GSH-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

L-Glutathione-BSA Kd around 10-8 M very high affinity and specificity

No crossreactivity with close competitors:

Competitors Affinity compared to GSH-BSA
Oxidized Glutathione-BSA 50 000 times lower no crossreactivity
L-Cysteine-BSA 50 000 times lower no crossreactivity
L-Glutamate-BSA 50 000 times lower no crossreactivity
Reduced Glutathione free 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Other Neurotransmitters

Isotype

IgG

Target

Conjugated L-Glutathione

Immunogen

Synthetic L-Glutathione (reduced) conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: L-Glutathione conjugate (Product number: AG047)

Related antibodies

Polyneuros also sells poly- and monoclonal antibodies against other molecules of the Glutathione pathway: anti-Glycine (AP028), anti-L-Glutamate (AP029 or AM003). Polyneuros sells as well antibodies against small molecules of the Scavengers & Vitamins sub-category: anti-D-alpha-Tocopherol succinate (AP058), anti-Retinoic acid (AP057), anti-Folic acid (AP099), anti-Uric acid (AP162)