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Retinoic acid antibody – AP057

Polyclonal anti-conjugated Retinoic acid antibody (rabbit)

SKU: AP057 Category:

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Additional information

Specifity

Using Retinoic acid-BSA conjugate (RA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Retinoic acid-BSA Kd around 10-8 M very high affinity and specificity

Low affinity with close competitors:

Competitors Affinity compared to RA-BSA
Palmitic acid-BSA 15 times lower low affinity
Farnesyl-Cysteine-BSA 25 times lower low affinity
Myristic acid-BSA 200 times lower low affinity
Oleic acid-BSA 350 times lower low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Target

Conjugated Retinoic acid (active metabolite of Vitamin A)

Immunogen

Synthetic all trans-Retinoic acid conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry (metabolic ways), Neurobiology (Growth and development, memory processes)

Applications

ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Retinoic acid conjugate (Product number: AG057)

Related antibodies

Polyneuros also sells the polyclonal antibodies against another molecule involved in stroke: anti-DL-Homocysteine antibody (AP035). Polyneuros also sells polyclonal antibodies against other molecules of the Scavengers & vitamins category: anti-Glutathione (AP047), anti-D-alpha-Tocopherol succinate (AP058), anti-Folic acid (AP099), anti-Uric acid (AP162)