Additional information
Specifity
Using D-alpha-Tocopherol succinate-BSA conjugate (alpha-TE-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
| D-Alpha-Tocopherol succinate-BSA | Kd around 10-8 M | very high affinity and specificity |
Low affinity with close competitors:
| Competitors | Affinity compared to alpha-TE-BSA | |
|---|---|---|
| Oleic acid-BSA | 100 times lower | low affinity |
| Myristic acid-BSA | 100 times lower | low affinity |
The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).
Additional Information
- Host species
Rabbit
- Clonality
Polyclonal
- Isotype
IgG
- Target
Conjugated D-alpha-Tocopherol succinate (active metabolite of Vitamin E)
- Immunogen
Synthetic D-alpha-Tocopherol succinate conjugated to bovine serum albumin (BSA)
- Species reactivity
The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule
- Purity
Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation
- Form
Lyophilized
- Stabilizers / preservatives
None
- Storage instructions and buffer
Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles
- Research areas
Biochemistry (metabolic ways), Neurobiology
- Applications
ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC
- Corresponding antigen/related product
To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: D-alpha-Tocopherol succinate conjugate (Product number: AG058)
- Related antibodies
Polyneuros also sells polyclonal antibodies against other molecules of the Scavengers & vitamins category: anti-Glutathione (AP047), anti-Retinoic acid (AP057), anti-Folic acid (AP099), anti-Uric acid (AP162)