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D-alpha-Tocopherol antibody – AP058

Polyclonal anti-conjugated D-alpha-Tocopherol succinate antibody (rabbit)

SKU: AP058 Category:

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Additional information

Specifity

Using D-alpha-Tocopherol succinate-BSA conjugate (alpha-TE-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

D-Alpha-Tocopherol succinate-BSA Kd around 10-8 M very high affinity and specificity

Low affinity with close competitors:

Competitors Affinity compared to alpha-TE-BSA
Oleic acid-BSA 100 times lower low affinity
Myristic acid-BSA 100 times lower low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Target

Conjugated D-alpha-Tocopherol succinate (active metabolite of Vitamin E)

Immunogen

Synthetic D-alpha-Tocopherol succinate conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry (metabolic ways), Neurobiology

Applications

ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: D-alpha-Tocopherol succinate conjugate (Product number: AG058)

Related antibodies

Polyneuros also sells polyclonal antibodies against other molecules of the Scavengers & vitamins category: anti-Glutathione (AP047), anti-Retinoic acid (AP057), anti-Folic acid (AP099), anti-Uric acid (AP162)