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Folic acid (vitamine B9) antibody – AP099

Polyclonal anti-conjugated Folic acid (vitamine B9) antibody (rat)

SKU: AP099 Category:

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Additional information

Specifity

Using Folic acid-BSA conjugate (FA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Folic acid-BSA Kd around 10-8 M very high affinity and specificity

Low affinity or no crossreactivity with close competitors:

Competitors Affinity compared to FA-BSA
Folinic acid-BSA 900 times lower low affinity
Tetrahydrofolic acid-BSA 2 000 times lower low affinity
Methotrexate-BSA 50 000 times lower no crossreactivity
Riboflavin-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rat

Clonality

Polyclonal

Isotype

IgG

Target

Conjugated Folic (Vitamine B9) acid

Immunogen

Synthetic Folic acid conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry (metabolic ways), Cardiovascular diseases, Neurobiology, Neurodegenerative diseases

Applications

ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Polyneuros sells as well the corresponding antigen: Folic acid conjugate (Product number: AG099)

Related antibodies

Polyneuros also sells the polyclonal antibodies against another molecule involved in stroke: anti-DL-Homocysteine antibody (AP035). Polyneuros also sells polyclonal antibodies against other molecules of the Scavengers & vitamins category: anti-Glutathione (AP047), anti-D-alpha-Tocopherol succinate (AP058), anti-Folic acid (AP099), anti-Uric acid (AP162)